tryple express trypsin protease Search Results


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Nectin-1 and HVEM are expressed in human oral epithelia and primary keratinocytes. (a) RNAs were isolated from epithelial sheets prepared from vestibular mucosa or from primary oral keratinocytes. RT-PCR demonstrates expression of nectin-1 and HVEM; as a control, GAPDH transcripts are shown. (b) Cells were dissociated from vestibular epithelial sheets by <t>TrypLE</t> <t>Select</t> treatment and stained for nectin-1. To investigate surface expression of HVEM, cells were dissociated from epithelial sheets by CDS treatment. Flow cytometric analyses indicate 92% and 40% of cells were positive for nectin-1 and HVEM, respectively. APC-A, allophycocyanin-conjugated antibody; FITC-A, fluorescein isothiocyanate-conjugated antibody. (c) Results from samples from 4 (nectin-1) or 3 (HVEM) different patients are shown as means ± standard deviation (SD), demonstrating that surface expression of HVEM is present on fewer cells than nectin-1.
Tryple Select, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nectin-1 and HVEM are expressed in human oral epithelia and primary keratinocytes. (a) RNAs were isolated from epithelial sheets prepared from vestibular mucosa or from primary oral keratinocytes. RT-PCR demonstrates expression of nectin-1 and HVEM; as a control, GAPDH transcripts are shown. (b) Cells were dissociated from vestibular epithelial sheets by <t>TrypLE</t> <t>Select</t> treatment and stained for nectin-1. To investigate surface expression of HVEM, cells were dissociated from epithelial sheets by CDS treatment. Flow cytometric analyses indicate 92% and 40% of cells were positive for nectin-1 and HVEM, respectively. APC-A, allophycocyanin-conjugated antibody; FITC-A, fluorescein isothiocyanate-conjugated antibody. (c) Results from samples from 4 (nectin-1) or 3 (HVEM) different patients are shown as means ± standard deviation (SD), demonstrating that surface expression of HVEM is present on fewer cells than nectin-1.
Tryple Express, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nectin-1 and HVEM are expressed in human oral epithelia and primary keratinocytes. (a) RNAs were isolated from epithelial sheets prepared from vestibular mucosa or from primary oral keratinocytes. RT-PCR demonstrates expression of nectin-1 and HVEM; as a control, GAPDH transcripts are shown. (b) Cells were dissociated from vestibular epithelial sheets by <t>TrypLE</t> <t>Select</t> treatment and stained for nectin-1. To investigate surface expression of HVEM, cells were dissociated from epithelial sheets by CDS treatment. Flow cytometric analyses indicate 92% and 40% of cells were positive for nectin-1 and HVEM, respectively. APC-A, allophycocyanin-conjugated antibody; FITC-A, fluorescein isothiocyanate-conjugated antibody. (c) Results from samples from 4 (nectin-1) or 3 (HVEM) different patients are shown as means ± standard deviation (SD), demonstrating that surface expression of HVEM is present on fewer cells than nectin-1.
Tryple Express Trypsin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nectin-1 and HVEM are expressed in human oral epithelia and primary keratinocytes. (a) RNAs were isolated from epithelial sheets prepared from vestibular mucosa or from primary oral keratinocytes. RT-PCR demonstrates expression of nectin-1 and HVEM; as a control, GAPDH transcripts are shown. (b) Cells were dissociated from vestibular epithelial sheets by <t>TrypLE</t> <t>Select</t> treatment and stained for nectin-1. To investigate surface expression of HVEM, cells were dissociated from epithelial sheets by CDS treatment. Flow cytometric analyses indicate 92% and 40% of cells were positive for nectin-1 and HVEM, respectively. APC-A, allophycocyanin-conjugated antibody; FITC-A, fluorescein isothiocyanate-conjugated antibody. (c) Results from samples from 4 (nectin-1) or 3 (HVEM) different patients are shown as means ± standard deviation (SD), demonstrating that surface expression of HVEM is present on fewer cells than nectin-1.
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Nectin-1 and HVEM are expressed in human oral epithelia and primary keratinocytes. (a) RNAs were isolated from epithelial sheets prepared from vestibular mucosa or from primary oral keratinocytes. RT-PCR demonstrates expression of nectin-1 and HVEM; as a control, GAPDH transcripts are shown. (b) Cells were dissociated from vestibular epithelial sheets by <t>TrypLE</t> <t>Select</t> treatment and stained for nectin-1. To investigate surface expression of HVEM, cells were dissociated from epithelial sheets by CDS treatment. Flow cytometric analyses indicate 92% and 40% of cells were positive for nectin-1 and HVEM, respectively. APC-A, allophycocyanin-conjugated antibody; FITC-A, fluorescein isothiocyanate-conjugated antibody. (c) Results from samples from 4 (nectin-1) or 3 (HVEM) different patients are shown as means ± standard deviation (SD), demonstrating that surface expression of HVEM is present on fewer cells than nectin-1.
Papain Worthington Ls003119 Tryple Express Thermo Fisher, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nectin-1 and HVEM are expressed in human oral epithelia and primary keratinocytes. (a) RNAs were isolated from epithelial sheets prepared from vestibular mucosa or from primary oral keratinocytes. RT-PCR demonstrates expression of nectin-1 and HVEM; as a control, GAPDH transcripts are shown. (b) Cells were dissociated from vestibular epithelial sheets by <t>TrypLE</t> <t>Select</t> treatment and stained for nectin-1. To investigate surface expression of HVEM, cells were dissociated from epithelial sheets by CDS treatment. Flow cytometric analyses indicate 92% and 40% of cells were positive for nectin-1 and HVEM, respectively. APC-A, allophycocyanin-conjugated antibody; FITC-A, fluorescein isothiocyanate-conjugated antibody. (c) Results from samples from 4 (nectin-1) or 3 (HVEM) different patients are shown as means ± standard deviation (SD), demonstrating that surface expression of HVEM is present on fewer cells than nectin-1.
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Nectin-1 and HVEM are expressed in human oral epithelia and primary keratinocytes. (a) RNAs were isolated from epithelial sheets prepared from vestibular mucosa or from primary oral keratinocytes. RT-PCR demonstrates expression of nectin-1 and HVEM; as a control, GAPDH transcripts are shown. (b) Cells were dissociated from vestibular epithelial sheets by <t>TrypLE</t> <t>Select</t> treatment and stained for nectin-1. To investigate surface expression of HVEM, cells were dissociated from epithelial sheets by CDS treatment. Flow cytometric analyses indicate 92% and 40% of cells were positive for nectin-1 and HVEM, respectively. APC-A, allophycocyanin-conjugated antibody; FITC-A, fluorescein isothiocyanate-conjugated antibody. (c) Results from samples from 4 (nectin-1) or 3 (HVEM) different patients are shown as means ± standard deviation (SD), demonstrating that surface expression of HVEM is present on fewer cells than nectin-1.
Tryple Express, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nectin-1 and HVEM are expressed in human oral epithelia and primary keratinocytes. (a) RNAs were isolated from epithelial sheets prepared from vestibular mucosa or from primary oral keratinocytes. RT-PCR demonstrates expression of nectin-1 and HVEM; as a control, GAPDH transcripts are shown. (b) Cells were dissociated from vestibular epithelial sheets by <t>TrypLE</t> <t>Select</t> treatment and stained for nectin-1. To investigate surface expression of HVEM, cells were dissociated from epithelial sheets by CDS treatment. Flow cytometric analyses indicate 92% and 40% of cells were positive for nectin-1 and HVEM, respectively. APC-A, allophycocyanin-conjugated antibody; FITC-A, fluorescein isothiocyanate-conjugated antibody. (c) Results from samples from 4 (nectin-1) or 3 (HVEM) different patients are shown as means ± standard deviation (SD), demonstrating that surface expression of HVEM is present on fewer cells than nectin-1.
Tryple Express Enzyme, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nectin-1 and HVEM are expressed in human oral epithelia and primary keratinocytes. (a) RNAs were isolated from epithelial sheets prepared from vestibular mucosa or from primary oral keratinocytes. RT-PCR demonstrates expression of nectin-1 and HVEM; as a control, GAPDH transcripts are shown. (b) Cells were dissociated from vestibular epithelial sheets by <t>TrypLE</t> <t>Select</t> treatment and stained for nectin-1. To investigate surface expression of HVEM, cells were dissociated from epithelial sheets by CDS treatment. Flow cytometric analyses indicate 92% and 40% of cells were positive for nectin-1 and HVEM, respectively. APC-A, allophycocyanin-conjugated antibody; FITC-A, fluorescein isothiocyanate-conjugated antibody. (c) Results from samples from 4 (nectin-1) or 3 (HVEM) different patients are shown as means ± standard deviation (SD), demonstrating that surface expression of HVEM is present on fewer cells than nectin-1.
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Image Search Results


Nectin-1 and HVEM are expressed in human oral epithelia and primary keratinocytes. (a) RNAs were isolated from epithelial sheets prepared from vestibular mucosa or from primary oral keratinocytes. RT-PCR demonstrates expression of nectin-1 and HVEM; as a control, GAPDH transcripts are shown. (b) Cells were dissociated from vestibular epithelial sheets by TrypLE Select treatment and stained for nectin-1. To investigate surface expression of HVEM, cells were dissociated from epithelial sheets by CDS treatment. Flow cytometric analyses indicate 92% and 40% of cells were positive for nectin-1 and HVEM, respectively. APC-A, allophycocyanin-conjugated antibody; FITC-A, fluorescein isothiocyanate-conjugated antibody. (c) Results from samples from 4 (nectin-1) or 3 (HVEM) different patients are shown as means ± standard deviation (SD), demonstrating that surface expression of HVEM is present on fewer cells than nectin-1.

Journal: Journal of Virology

Article Title: Mechanical Barriers Restrict Invasion of Herpes Simplex Virus 1 into Human Oral Mucosa

doi: 10.1128/JVI.01295-17

Figure Lengend Snippet: Nectin-1 and HVEM are expressed in human oral epithelia and primary keratinocytes. (a) RNAs were isolated from epithelial sheets prepared from vestibular mucosa or from primary oral keratinocytes. RT-PCR demonstrates expression of nectin-1 and HVEM; as a control, GAPDH transcripts are shown. (b) Cells were dissociated from vestibular epithelial sheets by TrypLE Select treatment and stained for nectin-1. To investigate surface expression of HVEM, cells were dissociated from epithelial sheets by CDS treatment. Flow cytometric analyses indicate 92% and 40% of cells were positive for nectin-1 and HVEM, respectively. APC-A, allophycocyanin-conjugated antibody; FITC-A, fluorescein isothiocyanate-conjugated antibody. (c) Results from samples from 4 (nectin-1) or 3 (HVEM) different patients are shown as means ± standard deviation (SD), demonstrating that surface expression of HVEM is present on fewer cells than nectin-1.

Article Snippet: Cell suspensions prepared by TrypLE Select (Life Technologies) were incubated in PBS–5% FCS on ice for 45 min with mouse anti-nectin-1 (CK41; 1:100) ( 40 ), and nectin-1 was visualized with anti-mouse IgG-Cy5 (1:100 [Jackson ImmunoResearch Laboratories, Inc.]).

Techniques: Isolation, Reverse Transcription Polymerase Chain Reaction, Expressing, Control, Staining, Standard Deviation